RT Journal Article
SR Electronic
T1 Immunoglobulin and T-cell receptor gene rearrangement
JF Cleveland Clinic Journal of Medicine
JO Cleve Clin J Med
FD Cleveland Clinic
SP 716
OP 721
VO 56
IS 7
A1 Fishleder, Andrew J.
YR 1989
UL http://www.ccjm.org/content/56/7/716.abstract
AB Recent studies have significantly broadened understanding of immunoglobulin production and T-cell receptor formation at the gene level. A limited number of genes can be rearranged during the course of B-cell or T-cell development to yield unique DNA sequences that will code for specific antibodies and T-cell receptor proteins. Southern blot hybridization analysis allows sensitive examination of lymphocyte DNA for the presence or absence of gene rearrangements. General mechanisms that underlie immunoglobulin and T-cell receptor rearrangement are reviewed, along with the diagnostic applications of detection of gene rearrangement by Southern blot hybridization technique.