@article {Cunningham69, author = {James R. Cunningham and Ronald M. Bukowski and Rafael Valenzuela and Raymond Tubbs}, title = {Immunologic studies of peripheral blood lymphocytes and lymph nodes in Hodgkin{\textquoteright}s disease}, volume = {52}, number = {1}, pages = {69--72}, year = {1985}, publisher = {Cleveland Clinic Journal of Medicine}, abstract = {Peripheral blood lymphocytes (PBL) and lymph nodes from patients with newly diagnosed and untreated Hodgkin{\textquoteright}s disease were studied to determine the distribution of T lymphocytes. Fluorescence-activated cell sorting of PBL was performed on 11 out of 12 patients using monoclonal antibodies to lymphocyte differentiation antigens. A reduction in T lymphocytes (anti-T3) was found in 5 patients, a reduction of T4 lymphocytes was found in 3 patients, and an increase in the PBL Tg cells occurred in 1 patient. In three cases, the T4:Tg PBL ratio was decreased. Complete staging was performed on all 12 patients. No correlation between clinical stage and T-cell subpopulations was apparent. Evidence of sustained immune depletion was found in 1 patient at relapse. Efforts to monitor immune function in Hodgkin{\textquoteright}s disease should continue and may provide a data base from which immunopathologic staging can be developed.}, issn = {0891-1150}, URL = {https://www.ccjm.org/content/52/1/69}, eprint = {https://www.ccjm.org/content/52/1/69.full.pdf}, journal = {Cleveland Clinic Journal of Medicine} }