RT Journal Article SR Electronic T1 Immunologic studies of peripheral blood lymphocytes and lymph nodes in Hodgkin’s disease JF Cleveland Clinic Journal of Medicine JO Cleve Clin J Med FD Cleveland Clinic SP 69 OP 72 VO 52 IS 1 A1 James R. Cunningham A1 Ronald M. Bukowski A1 Rafael Valenzuela A1 Raymond Tubbs YR 1985 UL http://www.ccjm.org/content/52/1/69.abstract AB Peripheral blood lymphocytes (PBL) and lymph nodes from patients with newly diagnosed and untreated Hodgkin’s disease were studied to determine the distribution of T lymphocytes. Fluorescence-activated cell sorting of PBL was performed on 11 out of 12 patients using monoclonal antibodies to lymphocyte differentiation antigens. A reduction in T lymphocytes (anti-T3) was found in 5 patients, a reduction of T4 lymphocytes was found in 3 patients, and an increase in the PBL Tg cells occurred in 1 patient. In three cases, the T4:Tg PBL ratio was decreased. Complete staging was performed on all 12 patients. No correlation between clinical stage and T-cell subpopulations was apparent. Evidence of sustained immune depletion was found in 1 patient at relapse. Efforts to monitor immune function in Hodgkin’s disease should continue and may provide a data base from which immunopathologic staging can be developed.